Protective Effect of Emodin against Airway Inflammation in the Ovalbumin-Induced Mouse Model

作     者：王坦 钟相根 李宇航 贾旭 张淑静 高誉珊 刘妙 吴若菡 

作者机构：School of Basic Medical SciencesBeijing University of Chinese Medicine 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2015年第21卷第6期

页      面：431-437页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the National Program on Key Basic Research Program Project(973 Program No.2009CB522704) 

主　　题：asthma emodin airway inflammation Chinese medicine 

摘      要：Objective： To investigate whether emodin exerts protective effects on mouse with allergic asthma. Methods： A mouse model of allergic airway inflammation was employed. The C57BL/6 mice sensitized and challenged with ovalbumin （OVA） were intraperitoneally administered 10 or 20 mg/kg emodin for 3 days during OVA challenge. Animals were sacrificed 48 h after the last challenge. Inflammatory cell count in the bronchoalveolar lavage fluid （BALF） was measured. The levels of interleukin （IL）-4, IL-5, IL-13 and eotaxin in BALF and level of immunoglobulin E （IgE） in serum were measured with enzyme-linked immuno sorbent assay kits. The mRNA expressions of IL-4, IL-5, home oxygenase （HO）-I and matrix metalloproteinase-9 （MMP- 9） were determined by real-time quantitative polymerase chain reaction. Results： Emodin induced significant suppression of the number of OVA-induced total inflammatory cells in BALF. Treatment with emodin led to significant decreases in the levels of IL-4, IL-5, IL-13 and eotaxin in BALF and total IgE level in serum. Histological examination of lung tissue revealed marked attenuation of allergen-induced lung eosinophilic inflammation. Additionally, emodin suppressed IL-4, IL-5 and MMP-9 mRNA expressions and induced HO-1 mRNA expression. Conclusion： Emodin exhibits anti-inflammatory activity in the airway inflammation mouse model, supporting its therapeutic potential for the treatment of allergic bronchial asthma.