Self-assembly of Human Galectin-1 via dual supramolecular interactions and its inhibition of T-cell agglutination and apoptosis

作     者：Wenjing Qi Yufei Zhang Zdravko Kochovski Jue Wang Yan Lu Guosong Chen Ming Jiang 

作者机构：The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science Fudan University Shanghai 200438 China Soft Matter and Functional Materials Helmholtz-Zentrum Berlin for Materialien und Energie Berlin 14109 Germany Institute of Chemistry University of Potsdam 14476 Potsdam Germany 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2018年第11卷第10期

页      面：5566-5572页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0903[农学-农业资源与环境] 0703[理学-化学] 0713[理学-生态学] 

基　　金：We acknowledge the financial support from the National Natural Science Foundation of China (Nos. 51721002  21504016  and 91527305). We thank Joint Lab for Structural Research at the Integrative Research Institute for the Sciences (IRIS Adlershof  Berlin) for Cryo-TEM imaging 

主　　题：protein self-assembly supramolecularinteractions galectin cell agglutination 

摘      要：Recently, we proposed a new strategy to construct artificial plant protein assemblies, which were induced by adding a small molecule, based on dual supramolecular interactions. In this paper, we further explored this method by employing Human Galectin-1 （Gal-1） as a building block to form self-assembled microribbons. Two non-covalent interactions, including lactose-lectin binding and dimerization of Rhodamine B （RhB）, induced by the small molecule ligand addition, were involved in the crosslinking of the animal protein, resulting in the formation of assemblies. By using transmission electron microscopy （TEM）, cryo-electron microscopy （cryo-EM）, and three-dimensional （3D） tomographic analysis, we arrived at a possible mechanistic model for the microribbon formation. Furthermore, the morphology of protein assemblies could be fine-tuned by varying the incubation time, the protein/ligand ratio, and the chemical structures of ligands. Interestingly, the formation of protein microribbons successfully inhibited Gal-1 induced T-cell agglutination and apoptosis. This is because the multivalent and dynamic interactions in protein assemblies compete with the binding between Gal-1 and the glycans on cell surfaces, which suppresses the function of Gal-1 in promotion of tumor progression and metastasis.