F-01A,an antibiotic,inhibits lung cancer cells proliferation

作     者：WANG Jing WU Xiao-Peng SONG Xin-Ming HAN Chang-Ri CHEN Zhong CHEN Guang-Ying 

作者机构：Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education Hainan Normal University College of Life Sciences Hainan Normal University Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2014年第12卷第4期

页      面：284-289页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 100706[医学-药理学] 1002[医学-临床医学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Nature Science Foundation of China(Nos.31360069,21162009) the Program for New Century Excellent Talents in University(No.NCET-08-0656) 

主　　题：Apoptosis Membrane potential SPC-A-1 cells Polyether antibiotic 

摘      要：AIM: In an effort to identify novel, small molecules which can affect the proliferation of lung cancer cells, F-01A, a polyether antibiotic isolated from the fermentation broth of Streptomyces was tested. METHOD: F-01A was tested for its antitumor properties on the lung cancer cell line SPC-A-1, at six doses(0.1, 0.5, 1, 2.5, and 5 μmol·L-1), using various cellular assays. Cell viability was measured by the MTT assay, Hochest 33258 was used to study nuclear morphology; DNA ladder and the loss of mitochondrial membrane potential were also evaluated. RESULTS: F-01A induces apoptosis against SPC-A-1 cells in a dose-dependent manner. The IC50 is 0.65 μmol·L-1, and the inhibition at 5 μmol·L-1 is 87.89%. Further, JC-1 staining indicates F-01A could induce the loss of mitochondrial membrane potential, and the DNA fragment is evident. CONCLUSION: Mechanistic analysis showed that F-01A induced apoptosis of cancer cells probably in the mitochondrial pathway. The antitumor actions of F-01A involve activation of the apoptotic pathway against SPC-A-1 cells, and it may be valuable for further drug development.