LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

作     者：Zhiyu ZHANG Changxiao LIU Duanyun SI Rong LU Xiulin YI 

作者机构：School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China State Key Laboratory of Drug Delivery Technology and PharmacokineticsTianjin Institute of Pharmaceutical ResearchTianjin 300193China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2012年第6卷第4期

页      面：436-442页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 083001[工学-环境科学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 10[医学] 

基　　金：New Drug Creation Project Foundation of China, (2011ZX09102-009-002, 2012ZX09304-002) National Basic Research Program of China (973 Program), (2010CB735602, 2012CB72400-2) 

主　　题：catalpol LC/MS/MS OCT2 

摘      要：More and more herbal medicines are found to be the substrates of drug transporters. In this paper, chromatography/tandem mass spectrometry （LC/MS/MS） combined with radioisotope method was used for the quantification of catalpol, a traditional Chinese medicine, to study the affinity relationship between herbal medicines and transporters. Catalpol uptake experiment was carried out by using several transporters （OAT1, OCT2, OAT3, OATP 1B 1 and OATP2B 1）. And samples were precipitated with methanol and quantified with LC/MS/MS. The results show that catalpol has a good affinity with OCT2- transfected $2 cells. After studying drug-drug interaction between catalpol and 14C-tetraethylammonium （TEA）, we found that catalpol is able to facilitate TEA transport mediated by OCT2, suggesting that catalpol could probably be a new promoter of OCT2.