Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin,an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

作     者：Li Zhi-neng Liu Guo-feng Fang Fang Bao Man-zhu 

作者机构：Key Laboratory of Horticultural Plant Biology Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 P. R. China 

出 版 物：《Forestry Studies in China》 (中国林学（英文版）)

年 卷 期：2007年第9卷第1期

页      面：14-18页

学科分类：0710[理学-生物学] 090701[农学-林木遗传育种] 0907[农学-林学] 07[理学] 08[工学] 0829[工学-林业工程] 09[农学] 071007[理学-遗传学] 

基　　金：This research is partially supported by the National Natural Science Foundation of China (Grant No. 30371015) the Bureau of Science and Technology of Wuhan. The kindness of doctor Lin Yong-jun in providing Agrobacterium tumefaciens strains EHA 105, harboring the binary vector pCAMBI2301 for this experiment is greatly appreciated. We thank all the colleagues in our laboratory, especially doctor Gao Li-ping, for constructive discussion and technical support 

主　　题：Platanus acerifolia Agrobacterium tumefaciens leaf explant plant regeneration genetic transformation antibiotic,Timentin 

摘      要：The effects of Timentin and cefotaxime （Cef） on shoot regeneration of the London plane tree （Platanus acerifolia Willd.） and their use for the suppression ofAgrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L^-1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L^-1; however, 300 mg·L^-1 Timentin was shown to facilitate shoot regeneration significantly and the regeneration frequency increased from 64% （control） to 88%. Effective suppression of A. tumefaciens could be obtained with 500 mg·L^-1 Cef, but plant regeneration was completely inhibited at this level. The A. tumefaciens on infected P. acerifolia leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L^-1 Timentin. Considering the effect of Cef and Timentin on plant regeneration and suppression of Agrobacteria, Timentin at 300 mg·L^-1 is the preferred application in .4. tumefaciens-mediated transformation ofP acerifolia.