Studies of CTLA4Ig in acute rejection of pancreas transplantation in rats
Studies of CTLA4Ig in acute rejection of pancreas transplantation in rats作者机构:Department of Pancreas Surgerythe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029 China
出 版 物:《Journal of Nanjing Medical University》 (南京医科大学学报(英文版))
年 卷 期:2006年第20卷第5期
页 面:263-265页
学科分类:1002[医学-临床医学] 100210[医学-外科学(含:普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金:the"135"Foundation of Jiangsu Province(2003-19)
主 题:pancreas transplantation rejection CTLA4Ig rats
摘 要:Objective: To investigate the protective effect of CTLA4Ig in rejection of pancreaticoduodenal transplantation model of rat. Methods : Pancreaticoduodenal transplantion models were established from the donor F344 rats to the Lewis recipients. The models were divided into 2 groups: Group A and B with 12 rats in each group.2 days after transplantation, reciepients in group A were treated with i.p. injection of sailine, and those in group B CTLA41 were injected(200μg). On day 1,4,7, 10 after transplantation, the grafts were harvested for histopathological examination. On day 4 after transplantation, the CD4^+CD25^+ T cells in the grafts were detected by Flow Cytometry. Results: Compared with group A: the degree of the rejection of grafts in group B was lower. The number of CD4^CD25^+ T cells of graft was (7.91±1.26)% in group A and (13.81±1.71)% in group B, which had significant difference (P〈0.01). Conclusion: CTLA4Ig could inhibit T cell costimulatory pathway, prevent acute rejection, which might be mediated bv increasing the number of CD4^+CD25^+ regulatory T cells.