Human Papillomavirus 16E6 Oncogene Mutation in Cervical Cancer

作     者：Feng Sun Xiao-qin Ha Tong-de Lv Chuan-ping Xing Bin Liu Xiao-zhe Cao 

作者机构：Department of Pathology The Second People＇s Hospital of Zhuhai Zhuhai 519020 China Department of General Laboratory Department of Pathology Lanzhou General Hospital of PLALanzhou 730050 China 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：2009年第21卷第2期

页      面：97-101页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by a grant from the 11th Five-year Plan Medical Science Fundation of PLA (No06MA084) 

主　　题：Cervical cancer Human papUlomavirus E6 gene Mutation 

摘      要：Objective： Cervical cancer （CC） is the second most common type of cancer in women worldwide, after breast cancer. High-risk human papillomaviruses （HR-HPVs） are considered to be the major causes of cervical cancer. HPV16 is the most common type of HR-HPVs and HPV16 E6 gene is one of the major oncogenes. Specific mutations are considered as dangerous factors causing CC. This study was designed to find mutations of HPV16 E6 and the relationship between the mutations and the happening of CC. Methods： The tissue DNA was extracted from 15 biopsies of CC. Part of HPV16 E6 gene （nucleotide 201-523） was amplified by polymerase chain reaction （PCR） from the CC tissue DNA. The PCR fragments were sequenced and analyzed. Results： The result of PCR showed that the positive rate of HPV16 E6 was 93.33% （14/13）. After sequencing ana analyzing, in the 13 out of 14 PCR fragments, 4 maintained prototype （30.77%）, 8 had a same 350G mutation （61.54%）, and 1 had a 249G mutation （7.69%）. Conclusion： This study suggest that there is a high infection rate of HPV in cervical cancer and most of the HPV16 E6 gene has mutations. Those mutations may have an association with the development of cervical cancer.