Ex vivo expansion of CD34^+ cells and immunocytes from umbilical cord blood

作     者：Yarning Wei Xiumei Lin Ping Mao Jiongcai Lan Yaming Wei;Xiumei Lin;Ping Mao;Jiongcai Lan

作者机构：Central Laboratory Affiliated Guangzhou First Municipal People＇s Hospital Guangzhou Medical College Guangzhou 510180 China Department of Hematology Affiliated Guangzhou First Municipal People＇s Hospital Guangzhou Medical College Guangzhou 510180 China Department of Blood Transfusion Affiliated Nanfang Hospital Southern Medical University Guangzhou 510515China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2006年第5卷第6期

页      面：412-415页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by a grant from Posteoctoral Sciences Foundation of China (No.2002031281) 

主　　题：cord blood stem cell ex vivo expansion T cell NK call DCs 

摘      要：Objective： Umbilical cord blood stem cell transplantation （CBSCT） has approached significant success in leukemia treatment, but it is associated with higher rates of delayed or failed engraftment and relapse. This may be caused by immature immune calls of umbilical cord blood. We try to expand stem/progenitor calls and T, NK, DC immunocytes from umbilical cord blood for transplantation and immunotherapy. Methods： CB MNCs were cultured and analyzed for progenitor/stem calls, immunocytes at day 0, 3, 7 and 14 by using flowcytometry. Results： The combinations of SCF, IL-3, IL-6, plus IL-2 or/and IL-4 showed significantly expanded results both for UCB MNCs and CD34^＋ cells. CD34^＋ percentage went up from fresh CB 1.6% to the highest group E （SCF＋IL-3, 6, 2, 4） 11.1%. The average expansion multiples of CD34^＋ calls at 7th culture days were from 10 to 50 （SCF＋IL-3, 6, 2, 4）. The CD3^＋ T cells was （18.7±4.3）% in fresh cord blood, and decreased sharply in the medium without cytokine, while markedly increased in groups with cytokines combination, in group B, E, G and F, their level were about 2 times of fresh control. The fresh UCB contained （3.6±1.9）% CD56^＋ calls, NK cells only were expanded in groups with IL-2. DCs markers CDla, CD80, CD83 and CD86 expressed a lower level at day 3 in all test groups, and then increased sharply in groups E, F and G with IL-4 cytokin at 7th culture days. Conclusion： T calls, NK cells and DCs as well as stem/progenitor calls could be expanded in the same medium from CB MNCs with the combinations of cytokines. The combination of SCF, IL-2, IL-3, IL-6 and IL-4 showed a balancaable expansion result of both CD34^＋ cells and immunocytes at 7th culture days.