Flow-injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled-Reagent-Release Technology

作     者：宋正华 张尼 

作者机构：Department of Chemistry Northwest University Xi'an Shaanxi 710069 China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2003年第21卷第2期

页      面：175-180页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 0703[理学-化学] 10[医学] 

基　　金：ProjectsupportedbytheNaturalScienceFoundationofEducationCommittee ShaanxiProvince China . 

主　　题：reserpine flow injection chemiluminescence medicine urine 

摘      要：A sensitive and rapid chemiluminescence (CL) flow injection with controlled reagent release technology for the determination of reserpine was proposed. The CL reagents, luminol and dichromate, used in this sensor, were all immobilized on anion exchange resin. Through injection of 100 μL of water, the reagents on the anion exchange resin column were eluted and in the presence of reserpine, the CL intensity was decreased, by which reserpine could be sensed. Reserpine was quantified by measuring the decrement of CL intensity, which was observed linear with the logarithm of reserpine concentration in the range of 1 0-500 0 ng/mL, and the limit of detection was 0 4 ng/mL (3 σ ) with a relative standard deviation of less than 3 0%. The proposed procedure was applied in the assay of reserpine in pharmaceutical preparation and biological fluids without any pre treatment process and with sampling frequencies of 72 times per hour.