In vitro Transcriptome Analysis of Two Chinese Isolates of Streptococcus suis Serotype 2

作     者：Dake Zhang Nan Du Sufang Ma Qingtao Hu Guangwen Lu Wei Chen Changqing Zeng 

作者机构：CAS Key Laboratory of Genomic and Precision MedicineBeijing Institute of GenomicsChinese Academy of Sciences CAS Key Laboratory of Pathogenic Microbiology and ImmunologyInstitute of MicrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2014年第12卷第6期

页      面：266-275页

核心收录：

学科分类：090603[农学-临床兽医学] 090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the CAS Key Laboratory of Pathogenic Microbiology and Immunology of China (Grant No. 2009CASPMI-007) to DZ the National Natural Science Foundation of China (Grant No. 81201700) to DZ 

主　　题：Streptococcus suis serotype 2 Transcriptome Novel transcripts s RNA Virulence factor 

摘      要：The Streptococcus suis serotype 2（S. suis 2） isolates 05ZYH33 and 98HAH33 have caused severe human infections in China. Using a strand-specific RNA-seq analysis, we compared the in vitro transcriptomes of these two Chinese isolates with that of a reference strain（P1/7）. In the89 K genomic island that is specific to these Chinese isolates, a toxin–antitoxin system showed relatively high levels of transcription among the S. suis. The known virulence factors with high transcriptional activity in these two highly-pathogenic strains are mainly involved in adhesion, biofilm formation, hemolysis and the synthesis and transport of the outer membrane protein. Furthermore,our analysis of novel transcripts identified over 50 protein-coding genes with one of them encoding a toxin protein. We also predicted over 30 small RNAs（s RNAs） in each strain, and most of them are involved in riboswitches. We found that six s RNA candidates that are related to bacterial virulence, including csp A and rli38, are specific to Chinese isolates. These results provide insight into the factors responsible for the difference in virulence among the different S. suis 2 isolates.