Introduction of exogenous wild soybean DNA into cultivated soybean and RAPD molecular verification

作     者：谢纬武 王斌 雷勃钧 李希臣 卢翠华 钱华 周思君 

作者机构：Institute of GeneticsChinese Academy of SciencesBeijing 100101China Biotechnology Research CenterHeilongjiang Academy of Agricultural SciencesHarbin 150086China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：1995年第38卷第10期

页      面：1195-1201页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

主　　题：soybean pollen tube channel introduction of exogenous DNA RAPD 

摘      要：The exogenous total DNA of the wild high-protein soybean was transferred to cultivated soybean through the pollen tube channel and the genomic variation of the transformed progeny was detected by the method of RAPD(Random Amplified Polymorphic DNA). Distinguished variations were found in one of the 7 transformed plants of the first generation(D_1),of which the traits of fruition,outward appearance,leaf shape and flower colour were almost identical with those of the recipient parent;of which grain weight,seed coat colour and stem strength were situated between the two parents;and there were greatly more pods per plant and 12.5% higher content of protein in seeds than that of the recipient *** the properties have been invariably inherited for 3 generations. Through RAPD analysis of the genomes of the donor,the recipient and the transformed progeny(D_3)as a group,DNA polyrnorphisms were found in amplified products by 24 of 150 *** results prove that the exogenous DNA caused the distinct variance of the *** authors infer that the homogeneous recombination of large exogenous DNA is the main cause for the variance.