Role of T-cell receptor V beta 8.3 peptide vaccine in the prevention of experimental autoimmune uveoretinitis

作     者：ZHANG Rui YANG Pei-zeng WU Chang-you JIN Hao-li LI Bing HUANG Xiang-kun ZHOU Hong-yan GAO Yang ZHU Lian-xiang Aize Kijlstra 

作者机构：Zhongshan Ophthalmic Centre Sun Yat-sen University Guangzhou 510060 China Department of Immunology Sun Yat-sen University Guangzhou 510089 China Division of Immunology Children＇s Hospital Boston Harvard Medical School Boston MA 02115 USA Animal Sciences Group Wageningen University and Research Centre Lelystad Eye Research Institute Maastricht and Department of Ophthalmology Academic Medical Centre University of Amsterdam the Netherlands 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2006年第119卷第9期

页      面：740-748页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：This study was supported by grants from Innovation Research Groups (No. 30321004) and Guangdong Famous Doctor Project (No. 2004  199) 

主　　题：uveoretinitis autoimmune T-cell receptor peptide vaccine 

摘      要：Background T-cell receptor （TCR） plays an important role in the development of autoimmune diseases. Recently, it was reported that immunization of animals with TCR peptide derived from the pathogenic cells could prevent autoimmune diseases. The aim of this study was to investigate whether vaccination with a synthetic peptide from the hypervariable region of TCR Vβ 8.3, an experimental autoimmune uveoretinitis （EAU）-associated gene, was able to prevent the disease. Methods EAU was induced in Lewis rats by immunization with IRBP R16 peptide emulsified in complete Freund＇s adjuvant （CFA）. The clinical and histological appearances were scored. Delayed type hypersensitivity （DTH） and lymphocyte proliferation were detected. Cytokine levels of aqueous humour, supernatants of cells from spleen and draining lymph nodes were measured by enzyme linked immunosorbent assay （ELISA）. Gene expression of TCR Vβ8.3 on CD4^＋ T cells was examined by real time quantitative polymerase chain reaction （PCR）. Results After vaccination, the intraocular inflammation was significantly mitigated, antigen specific DTH and lymphocyte proliferation responses were suppressed, interleukin （IL）-2 in aqueous humour, interferon （IFN）-γ and IL-2 produced by the spleen and draining lymph node cells were significantly decreased, whereas the production of IL-4 and IL-10 were increased. The response of draining lymph node cells to TCR Vβ 8.3 peptide was enhanced after vaccination. Inoculation with CFA alone did not affect the severity of EAU and the above parameters. The suppression of EAU was much stronger in the group of four fold inoculations than the group of two fold inoculations. The expression of TCR Vβ 8.3 gene was significantly reduced in the group of fourfold inoculations. Conclusion Vaccination with the synthetic TCR Vβ 8.3 peptide could remarkably inhibit the development of EAU.