Fluorescence study on the interaction between apoCopC and cupric
Fluorescence study on the interaction between apoCopC and cupric作者机构:Institute of Molecular Science Chemical Biology and Molecular Engineering Laboratory of Ministry of Education Shanxi University Taiyuan 030006 China
出 版 物:《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)
年 卷 期:2005年第50卷第20期
页 面:2302-2305页
核心收录:
学科分类:081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学]
基 金:supported by the National Natural Science Foundation of China(Grant No.20371031) the Natural Science Foundation of Shanxi Province(Grant No.20031017)
摘 要:The interaction between apoCopC and cupric was investigated by fluorescence spectra, in phosphate (20 mmol/L) buffer at pH 6.0. Results suggest that the environ- ment is measured to be hydrophobic completely around tryptophan (83). At the same time, apoCopC fluorescence at 320 nm was significantly quenched with the addition of cu- pric and the 1:1 stoichiometric ratio of apoCopC to cupric was confirmed by fluorescence. In addition, the conditional binding constants were calculated to be KCu-Copc = (1.8±0.58)× 1013 mol?1 L on the basis of the results of fluorescence titra- tion curves. The apoCopC has the ability to bind specifically cupric ion.