Diosgenin induces apoptosis in HeLa cells via activation of caspase pathway

作     者：Shin-ichi TASHIRO,Satoshi ONODERA,Takashi IKEJIMA 

作者机构：China Department of Clinical and Biomedical Sciences Department of Clinical and Biomedical Sciences China-Japan Research Institute of Medical and Pharmaceutical Sciences Showa Pharmaceutical University Tokyo 194-8543 Japan Showa Pharmaceutical University Tokyo 194-8543 Japan Shenyang Pharmaceutical University 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第8期

页      面：103-108页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：diosgenin apoptosis caspases mitochondria HeLa cells 

摘      要：AIM: To investigate the mechanism of diosgenin-induced HeLa cell apoptosis. METHODS: HeLa cell growth was measured by MTT method. Apoptosis was detected by electron microscopy and agarose gel electrophoresis. Ratio of apoptotic cells was measured by APO-BRDU kit. Cell cycle distribution and changes of mitochondrial membrane potential were monitored by flow cytometry. Caspase activities were assayed by caspase apoptosis detection kit. Western blot analysis was used to evaluate the level of mitochondrial Bcl-2 expression. RESULTS: Diosgenin inhibited HeLa cell growth. HeLa cells treated with diosgenin showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-9 inhibitor (Ac-AAVALPAVLLALLAPLEHD-CHO), and caspase-3 inhibitor (z-DEVD-fmk) partially prevented diosgenin-induced apoptosis, but not caspase-8 inhibitor (z-IETD-fmk) and caspase-10 inhibitor (z-AEVD-fmk). Diosgenin caused reduction of mitochondrial membrane po