Effect of Nitrate on Activities and Transcript Levels of Nitrate Reductase and Glutamine Synthetase in Rice

作     者：CAO Yun FAN Xiao-Rong SUN Shu-Bin XU Guo-Hua HU Jiang SHEN Qi-Rong 

作者机构：College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 （China）. 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2008年第18卷第5期

页      面：664-673页

核心收录：

学科分类：0818[工学-地质资源与地质工程] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 

基　　金：the National Natural Science Foundation of China (Nos.30390082 and 40471074) 

主　　题：ammonium glutamine synthetase nitrate nitrate reductase Oryza sativa L. 

摘      要：Real-time polymerase chain reaction analysis was used to compare the effect of NO3^- on the activities of nitrate reductase （NR） and glutamine synthetase （GS）, and the transcript levels of two NR genes, OsNial and OsNia2, two cytosolic GS1 genes, OsGln1;1 and OsGln1;2, and one plastid GS2 gene OsGln2, in two rice （Oryza sativa L.） cultivars Nanguang （NG） and Yunjing （Y J）. Both cultivars achieved greater biomass and higher total N concentration when grown in a mixed N supply than in sole NH4^＋ nutrition. Supply of NO3^- increased NR activity in both leaves and roots. Expression of both NR genes was also substantially enhanced and transcript levels of OsNia2 were significantly higher than those of OsNial. NO3 also caused an increase in GS activity, but had a complex effect on the expression of the three GS genes. In roots, the OsGln1;1 transcript increased, but OsGln1;2 decreased. In leaves, NO3^- had no effect on the GS1 expression, but the transcript for OsGln2 increased both in the leaves and roots of rice with a mixed supply of N. These results suggested that the increase in GS activity might be a result of the complicated regulation of the various GS genes. In addition, the NO3-induced increase of biomass, NR activity, GS activity, and the transcript levels of NR and GS genes were proportionally higher in NG than in Y J, indicating a stronger response of NG to NO3^- nutrition than YJ.