Effect of methotrexate combined with Sanhuang Yilong decoction on serum and synovial fluid aquaporin levels in rheumatoid arthritis dampness-heat blockage syndrome

作     者：Liu Defang Yan Jiao Guo Mingyang Luo Yong Yang Mei Yang Min Zou Longfu Li Wan Zhong Xiaolong Liu Tao Hu Yonghe 

作者机构：Department of Integrative Medicine on Rheumatoid General Hospital of Chengdu Military Region Chengdu 610083 China Second Military Medical University Shanghai 200433 China Department of Central laboratory of General Hospital of Chengdu Military Region Chengdu 610083 China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2018年第38卷第4期

页      面：618-624页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：the Army Medical Research Twelfth Five Year Plan Key Project(No.BWS11J06) the Sichuan Provincial Health Department(No.120573) 

主　　题：Arthritis rheumatoid Dampness heat Aquaporins Blood sedimentation C-reactive pro-tein Sanhuang Yilong decoction 

摘      要：OBJECTIVE:To study the effect of methotrexate(MTX) combined with Sanhuang Yilong decoction(SYD) on aquaporin(AQP) expression,and to explore the role of AQPs in the pathogenesis of rheumatoid arthritis(RA).METHODS:A total of 118 dampness-heat blockage type RA patients who were hospitalized in the General Chengdu Military Hospital between January2014 and December 2016 were selected as subjects in this study(30 patient of these patients with knee joint effusion were assigned to the RA synovial fluid group).For the pre-treatment controlgroups,30 healthy volunteers were recruited as the healthy control group and 30 osteoarthritis(OA) patients with knee joint effusion were included as OA synovial fluid control *** RA dampnessheat blockage syndrome treatment groups were divided into 45 cases in the combined group and 45 cases in the MTX *** combined group received MTX combined with SYD treatment while the MTX group received MTX ***1,AQP2 and AQP3 expressions were detected in the serum and synovial ***:AQP1 had the highest expression,followed by AQP3,and *** serum levels of AQP1,AQP2 and AQP3 were all significantly lower than those in the healthy volunteers(P 0.05).After treatment for2 weeks,serum AQP1,AQP2,AQP3 were significantly increased and erythrocyte sedimentation rate,C-reactive protein,disease activity score of 28 joints were decreased in the combined group(P 0.05).CONCLUSION:Abnormal expression of AQPs inhibits water metabolism in RA dampness-heat blockage syndrome,so liquid is accumulated at the joint,which may play an important role in the pathogenesis of *** combined with SYD for the treatment of RA can effectively increase AQP expression.