Evaluation of the Hydroxynitrile Lyase Activity in Cell Cultures of Capulin (Prunus serotina)

作     者：Liliana Hemandez Hector Luna Arturo Navarro-Ocafia Ma Teresa de Jesus Olivera-Flores Ivon Ayala 

作者机构：UniversidadAutdnoma Metropolitana UnidadXochimilco Depto. Sistemas Biologicos A. P. 23/181 Mgxico D. F. Mexico Facultad de Qufmica Universidad Nacional Autdnoma de Mexico Ciudad Universitaria Mgxico D. F. 04510 Mexico 

出 版 物：《生物工程学报》 (Chinese Journal of Biotechnology)

年 卷 期：2008年第24卷第7期

页      面：1199-1201页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0821[工学-纺织科学与工程] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 082101[工学-纺织工程] 090102[农学-作物遗传育种] 

主　　题：细胞 裂解酶 活性 柳叶野黑桃 

摘      要：Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The threeweek old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.