Rapid Detection of rpoB Gene Mutations in Rif-resistant *** Isolates by Oligonucleotide Microarray

作     者：AI-HUA SUN XING-LI FAN LI-WEI LI LI-FANG WANG WEN-YING AN JIE YAN 

作者机构：Department of Basic Medicine Zhejiang Medical College Hangzhou 310053 Zhejiang China Department of Medical Microbiology and Parasitology Zhejiang University Hangzhou 310058 Zhejiang China School of lnternational Stud3 Zhejiang University Hangzhou 310058 Zhejiang China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2009年第22卷第3期

页      面：253-258页

核心收录：

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the grant from the National Natural Science Foundation of China (No. 30400018) 

主　　题：Mycobacterium tuberculosis Rifampin resistance rpoB gene / site mutation Oligonucleotide microarray/detection 

摘      要：Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray Results Of the 102 rifampin-resistant strains 98 （96.1%） had mutations in the rpoB genes. Conclusion Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.