Protection of Immuno-Compromised Mice from Lethal Infection of Klebsiella pneumonia by rAAV2-BPI23-Fcγ1 Gene Transfer

作     者：Jing Li Qingli Kong Zhe Lv Yuanzhi Guan Yong Qiu Chen Li Mingjie Sun Zhenlong Liu Yunqing An 

作者机构：Department of Immunology Capital Medical University Beijing 100069China Laboratory Department Capital Institute of Pediatrics Beijing 100020China Department of Microbiology Institute of Basic Medical Sciences ChineseAcademy of Medical Sciences ＆ Peking Union Medical College Beijing100005 China Laboratory Department Beijing Shijitan Hospital Beijing 100038 China China Rehabilitation Center Institute of Medical Sciences Beijing 100077China 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2008年第5卷第6期

页      面：439-445页

核心收录：

学科分类：0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：funded by the Beijing Municipal Natural Science Foundation (7082016) 

主　　题：rAAV2 BPI immuno-compromised mouse Klebsiella pneumonia gene transfer 

摘      要：In previous research, chimerical BPI23-Fcy1 gene which consisted of human bactericidal/permeability increasing protein （BPI） gene of encoding the functional N terminus （amino acid residues 1 to 199） of human BPI and Fcy1 gene of encoding the Fc segment of human immunoglobulin G1 was successfully reconstructed within a recombinant adeno-associated virus serotype 2 （rAAV2） vector as rAAV2-BPI23-Fcy1. Here, to evaluate the potentiality of applying gene therapy to gram negative bacterial （GNB） infection in high-risk patients, we investigated protection of immuno-compromised mice and immunocompetent mice from challenge with minimal lethal dose （MLD） Klebsiella pneumonia infection after rAAV2-BPI23-Fcy1 gene transferred. The results showed that the survival rate of rAAV2-BPI23-Fcy1 transferred immunocompetent mice as well as immuno-compromised mice （40.0% and 44.4%, respectively） were significant higher than that of corresponding control mice （6.7% and 4.4%, respectively）; the bacteria counting, level of endotoxin and proinflammatory cytokines in the rAAV2-BP123-Fcy1 transferred immuno-compromised mice were markedly lower than that of rAAV2-EGFP and rAAV2-Null transferred immuno- compromised mice. Our data suggest that rAAV2-BPI23-Fcy1 gene transferring offered immuno-compromised mice with resistance against GNB infection, so it is quite potential in preventing GNB infection of clinical high-risk patients. Cellular ＆ Molecular Immunology. 2008;5（6）：439-445.