Vascular endothelial growth factor gene transfection to enhance the repair of avascular necrosis of the femoral head of rabbit

作     者：杨操 杨述华 杜靖远 李进 许伟华 熊宇芳 

作者机构：Department of OrthopedicsUnion HospitalTongji Medical CollegeHuazhong University of Science & Technology Department of BiochemistryTongji Medical CollegeHuazhong University of Science & Technology 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2003年第116卷第10期

页      面：1544-1548页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 071007[理学-遗传学] 10[医学] 

基　　金：ThisstudywassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 3 0 170 945 ) 

主　　题：vascular endothelial growth factor gene transfection avascular necrosis femoral head 

摘      要：Objective To explore a new method for the therapy of avascular necrosis of the femoral head. Methods The recombinant plasmid pCD-hVEGF 165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was examined by RNA dot hybridization and immunohistochemical techniques. Repair of the femoral head was observed by histological and histomorphometric *** The expression of VEGF was detected in the femoral head transfected with the VEGF gene. The femoral head transfected with the VEGF gene showed a significant increase in angiogenesis 2 and 4 weeks after gene transfection and a significant increase in bone formation 6 and 8 weeks after gene transfection on histomorphometric analysis ( P 0.01).Conclusions Transfection of the VEGF gene enhances bone tissue angiogenesis. Repair of osteonecrosis could be accelerated accordingly,thus providing a potential method for therapy of osteonecrosis.