Constant serum levels of secreted asialoglycoprotein receptor sH2a and decrease with cirrhosis

作     者：Ron Benyair Maria Kondratyev Elena Veselkin Sandra Tol-chinsky Marina Shenkman Yoav Lurie Gerardo Z Lederkremer 

作者机构：Department of Cell Research and Immunology George Wise Faculty of Life Sciences Tel Aviv University Liver Unit Department of Gastroenterology Tel Aviv Sourasky Medical Center 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2011年第17卷第48期

页      面：5305-5309页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by The Ramot research fund the Israel Science Foundation the Israel Cancer Association and the Israeli Ministry of Health (to Lederkremer GZ) 

主　　题：Asialoglycoprotein receptor Hepatitis C vi-rus Liver function Cirrhosis Liver diagnosis 

摘      要：AIM: To investigate the existence and levels of sH2a, a soluble secreted form of the asialoglycoprotein receptor in human serum. METHODS: Production of recombinant sH2a and development of a monoclonal antibody and an enzyme-linked immunosorbent assay (ELISA). This assay was used to determine the presence and concentration of sH2a in human sera of individuals of both sexes and a wide range of ages. RESULTS: The recombinant protein was produced successfully and a specific ELISA assay was developed. The levels of sH2a in sera from 62 healthy individuals variedminimally (147 ± 19 ng/mL). In contrast, 5 hepatitis C patients with cirrhosis showed much decreased sH2a levels (50 ± 9 ng/mL). CONCLUSION: Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals. This constant level and the decrease with cirrhosis suggest a diagnostic potential.