Waxy allelic diversity in common millet(Panicum miliaceum L.) in China

作     者：Ruiyun Wang Haigang Wang Xiaohuan Liu Xu Ji Ling Chen Ping Lu Minxuan Liu Bin Teng Zhijun Qiao 

作者机构：College of Agriculture Shanxi Agricultural University Institute of Crop Germplasm Resources of Shanxi Academy of Agricultural Sciences Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau Ministry of Agriculture Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops Institute of Crop Science Chinese Academy of Agricultural Sciences Anhui Provincial Key Lab of Rice Genetics and Breeding Institute of Rice Research Anhui Academy of Agricultural Sciences 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2018年第6卷第4期

页      面：377-385页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by the National Natural Science Foundation of China(31271791) Research Project supported by Shanxi Scholarship Council of China(2016-066) the Subject Construction Project of Anhui Academy of Agricultural Sciences(16A0102) China Agriculture Research System(CARS-06-13.5-A16) the Key Research and Development Program(General Project)(Agriculture)of Shanxi Province of China(201603D221003-5) the Special Program of Crop Germplasm Resources Protection and Utilization of Ministry of Agriculture of the People's Republic of China(2017NWB036-21) the Subplatform Project of National Proso Millet Germplasm Resources of China(NICGR2017-027) 

主　　题：基因 植物学 双波长比色法 中国 

摘      要：A set of 132 accessions of common millet,Panicum miliaceum L.,from 12 provinces of China were assessed for endosperm starch type(waxy or non-waxy)using I_2-KI staining,amylose and amylopectin contents using the dual-wavelength colorimetric method,and genotype of Waxy genes conditioning amylose content by gene sequencing.Endosperm starch content varied from57.69%to 74.70%,while the amylose and amylopectin contents of the starch ranged from 0 to23.29%and from 41.99%to 70.24%,respectively.Sequencing two Wx genes,including Wx-L(intron 5-exon 7 and intron 8–9)and Wx-S(exon 9–intron 10)revealed several polymorphisms(S_0,S_(-15),L_C,L_F,L_Y).Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones.Among the 132 accessions,68 with the S_(-15)allele had waxy endosperm starch with the amylose content range 0–2.58%and 64 accessions with the S_0allele had non-waxy endosperm starch with amylose content range 3.94%–23.29%.Five genotypes including S_(-15)/L_F(45%of the accessions),S_0/L_F(25%),S_0/L_Y(12%),S_0/L_C(11%),and S_(-15)/L_Y(7%)were identified.Six new SNPs were detected at the Wx-L locus.These results will facilitate common millet breeding,especially of cultivars free of amylose.