Cloning of Ds MAPK Gene and Construction of Antisense Expression Vector

作     者：Jinrong YUE Yuting CONG Xiangnan GAO Zhenyu XING Wenjing YUE Xiaojie CHAI 

作者机构：Dalian Ocean University/Key Laboratory of Maricuhure&Stock Enhancement in North China's SeaMinistry of Agricuhure/Key Laboratory of Marine Bio-Resources Restoration and Habitat Reparation in Liaoning ProvinceDalian 116023China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2018年第7卷第4期

页      面：8-11,24页

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by National Natural Science Foundation of China(31472260 30972240) 

主　　题：Dunaliella salina Ds MAPK Antisense expression vector Semi-quantitative RT-PCR 

摘      要：In order to investigate the role of MAPK gene in adaptation of Dunaliella salina to hypersaline environment, the Ds MAPK gene of D. salina was amplified by PCR. After inverted insertion of the open reading flame ofDs MAPK gene into downstream sequence of 35S promoter of plant expression vector, an antisense expression vector of Ds MAPK gene was successfully constructed and introduced into D. salina cells by LiAc/PEG-mediated method. The expression of Ds MAPK gene in transgenic D. salina was analyzed by semi-quantitative RT-PCR method. The results showed that the expression of Ds MAPK gene in D. salina was significantly inhibited at the transcriptional level. The study laid the foundation for further identification of the function of Ds MAPK gene.