Glycyrrhizinate reduces portal hypertension in isolated perfused rat livers with chronic hepatitis
Glycyrrhizinate reduces portal hypertension in isolated perfused rat livers with chronic hepatitis作者机构：Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College School of Basic Medicine Beijing University of Chinese Medicine
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2013年第19卷第36期
页 面：6069-6076页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by The National Natural Science Foundation of China,No.30873464 the Research Foundation from Ministry of Education of China,No.108019 the Natural Science Foundation of Beijing,China,No.7132150
主 题：Chronic hepatitis Portal hypertension Isolated portal perfused rat liver Diammonium glycyrrhizinate Inducible nitric oxide synthase
摘 要：AIM:To investigate the effects of diammonium glycyrrhizinate(Gly)on portal hypertension(PHT)in isolated portal perfused rat liver(IPPRL)with carbon tetrachloride(CCl4)-induced chronic ***:PHT model was replicated with CCl4 in rats for 84 *** was identified by measuring the ascetic amounts,hepatic function,portal pressure in vivo,splenic index,and pathological *** nitric oxide synthase(iNOS)in liver was assessed by *** were performed at d0,d28,d56,and *** phenylephrine-induced constriction,Gly was geometrically used to reduce *** action was expressed as median effective concentration(EC50)and area under the curve(AUC).Underlying mechanism was exploited by linear correlation between AUC values of Gly and existed iNOS in portal ***:PHT model was confirmed with ascites,splenomegaly,serum biomarkers of hepatic injury,and elevated portal *** findings had shown normal hepatic structure at d0,degenerations at d28,fibrosis at d56,cirrhosis at d84in PHT *** lobule ratios decreased and collagen ratios increased progressively along with PHT *** does dose-dependently reduce PHT in IPPRLs with CCl4-induced chronic *** potencies were increased gradually along with PHT development,characterized with its EC50at 2.80×10-10,3.03×10-11,3.77×10-11and 4.65×10-11mol/L at d0,d28,d56and d84,*** iNOS was located at hepatocyte at d0,stellate cells at d28,stellate cells and macrophages at d56,and macrophages in portal triads at *** infiltrated more into portal triads and expressed more iNOS along with PHT *** values of Gly were positively correlated with existed iNOS levels in portal ***:Gly reduces indirectly PHT in IPPRL with CCl4-induced chronic *** underlying mechanisms may relate to rescue NO bioavailability from macrophage-derived peroxynitrite in portal triads.
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