Effect of icariin on apoptosis in hippocampal neurons cultured in high glucose

作     者：Yan Bin Wang Jingbo Zhang Hong Tian Guoqing Liu Yuqin 

作者机构：Department of Traditional Chinese MedicinePeking Union Medical College HospitalChinese Academy of Medical Sciences Institute of Basic Medical SciencesChinese Academy of Medical Sciences 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2018年第38卷第4期

页      面：556-561页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Capital's Funds for Health Improvement and Research(No.SF2016-2-4019) 

主　　题：Icariin Protein kinase B High glucose Hippocampal neurons Apoptosis 

摘      要：OBJECTIVE:To investigate effect of icariin on apoptosis in hippocampal neurons cultured in high glucose,and the possible mechanism behind the ***:Hippocampus was obtained from newborn 24 h Sprague-Dawley rats and then primarily *** hippocampal neurons were divided into normal control group,high glucose group,icariin group,icariin + protein kinase B(Akt) inhibitor group and Akt agonist *** each group was cultured in different conditioned medium for 72 h,we detected the apoptosis of neurons with flow cytometry,and the expression of Akt,p-Akt,B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax) with western ***:Compared with the normal control group,the apoptosis rate of hippocampal neurons increased significantly(P 0.01),and p-Akt/Aktand Bcl-2/Bax decreased significantly(P 0.01) in high glucose *** with the high glucose group,the apoptosis rate of hippocampal neurons decreased significantly(P 0.01),and p-Akt/Akt and Bcl-2/Bax increased significantly(P 0.05)in icariin group and Akt agonist *** with the icariin + Akt inhibitor group,the apoptosis rate of hippocampal neurons decreased significantly(P 0.01),and p-Akt/Akt and Bcl-2/Bax increased significantly(P 0.01) in icariin ***:Icariin could reduce the apoptosis of neurons cultured in high glucose,which may be achieved by increasing the phosphorylation of Akt protein in Akt signal pathway,then increasing the expression of Bcl-2 and inhibiting the expression of Bax.