Genome-wide gene expression analysis for target genes to differentiate patients with intestinal tuberculosis and Crohn’s disease and discriminative value of FOXP3 mRNA expression

作     者：Vineet Ahuja Swati Subodh Amit Tuteja Veena Mishra Sushil Kumar Garg Neha Gupta Govind Makharia SK Acharya 

作者机构：Department of Gastroenterology and Human NutritionAll India Institute of Medical SciencesNew DelhiIndia The Centre for Genomic Application(An IGIB–IMM collaboration)New DelhiIndia 

出 版 物：《Gastroenterology Report》 (胃肠病学报道（英文）)

年 卷 期：2016年第4卷第1期

页      面：59-67,I0003页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This project was undertaken under the‘FIST’scheme of Department of Science and Technology Government of India.In addition support was taken from ICMR Senior Research Fellowship granted to VM 

主　　题：Crohn’s disease intestinal tuberculosis microarray gene expression profiling signaling pathway FOXP3 mRNA 

摘      要：Background and aims:Crohn’s disease(CD)and intestinal tuberculosis(ITB)are both chronic granulomatous conditions with similar phenotypic ***,there is need for a biomarker to differentiate between both these two *** study aimed at genome-wide gene expression analysis of colonic biopsies from confirmed cases of ITB and CD in comparison with *** evaluate the role of T regulatory cells,forkhead box P3(FOXP3)mRNA expression was quantified in serum as well as in colonic biopsies from patients with ITB and with the ***:Paired samples,including serum and colonic biopsies,were taken from 33 study subjects(CD,ITB and controls),and total RNA was *** whole genome gene expression microarray analysis was performed using the Illumina HumanWG-6 BeadChip Kit with six total RNA samples of the three groups in ***-time PCR for FOXP3 mRNA expression was analyzed in serum samples and colonic biopsy samples(4-CD,5-ITB,4-controls).Results:In CD and ITB there was 1.5-fold upregulation of 92 and 382 genes and 1.5-fold downregulation of 91 and 256 genes,*** proliferators via the PPARc pathway were most significantly downregulated(P2-fold *** ITB,the complement activation pathway,specifically the classical pathway,was the most significantly ***3 mRNA expression was significantly elevated in colonic biopsies obtained from ITB patients as compared with CD cases(4.7062.21 vs 1.4860.31,P=0.016).Conclusions:FOXP3 mRNA expression in colonic mucosa could be a discriminatory marker between ITB and *** of the complement activation pathway in ITB suggests that pathogenetic mechanisms for ITB are similar to those of pulmonary *** CD,downregulation of PPARc was seen in colonic tissue,suggesting that restoratio