Immune Responses in Wild-type Mice Against Prion Proteins Induced Using a DNA Prime-Protein Boost Strategy

作     者：HAN YanLing LI Yuan SONG Juan WANG Ying SHI Qi CHEN Cao ZHANG BaoYun GUO Yan LI ChaoPing HAN Jun DONG XiaoPing 

作者机构：State Key Laboratory for Infectious Disease Prevention and ControlNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Medical SchoolAnhui University of Science and TechnologyHuainan 231001China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2011年第24卷第5期

页      面：523-529页

核心收录：

学科分类：090601[农学-基础兽医学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by Chinese National Natural Science Foundation Grants 30771914 and 30800975 Institution Technique R&D Grant (2008EG150300) National Basic Research Program of China (973 Program) (2007CB310505) China Mega-Project for Infectious Disease (2009ZX10004‐101, 2008ZX10004‐001, and 2008ZX10004‐002) the SKLID Development Grant (2008SKLID102 and 2008SKLID202) 

主　　题：Prion DNA vaccine Humoral response T‐cell response Prime‐boosting regime. 

摘      要：Objective To break immune tolerance to prion （PrP） proteins using DNA *** Four different human prion DNA vaccine candidates were constructed based on the pcDNA3.1 vector：PrP‐WT expressing wild‐type PrP,Ubiq‐PrP expressing PrP fused to ubiquitin,PrP‐LII expressing PrP fused to the lysosomal integral membrane protein type II lysosome‐targeting signal,and PrP‐ER expressing PrP locating the *** a prime‐boost strategy,three‐doses of DNA vaccine were injected intramuscularly into Balb/c mice,followed by two doses of PrP *** weeks after the last immunization,sera and spleens were collected and PrP‐specific humoral and cellular immune responses evaluated by ELISA and ELISPOT *** Higher levels of serum PrP antibodies were detected in mice vaccinated using the strategy of DNA priming followed by protein *** these,WT‐PrP,Ubiq‐PrP,and PrP‐LII induced significantly higher humoral *** tests showed markedly increased numbers of IFN‐γ‐secreting T cells in mice vaccinated using the strategy of DNA priming followed by protein boosting after stimulation with recombinant PrP23‐90 and PrP23‐***‐ER inducedthe strongest T‐cell *** Prion vaccines can break tolerance to PrP proteins and induce PrP‐specific humoral and cellular immune responses.