Effects of Na^+/K^+ Ratio of Groundwaters on the Gill Ion-Transport Enzyme Activity,Plasma Osmolality and Growth of Cynoglossus semilaevis Juveniles

作     者：YANG Huizan PAN Luqing HU Fawen LIU Hongyu 

作者机构：The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2008年第7卷第4期

页      面：447-452页

学科分类：081803[工学-地质工程] 08[工学] 0818[工学-地质资源与地质工程] 

基　　金：the Agricultural Science and Technology Achievement Transfer Project of Tianjin City of China (Transfer of the Large-scale health culture technology of fish and shrimp) the Science and Technology Program of Shengli Oil Field Administration Bureau (The exploring study on the culture technology of fish and shrimp using saline groundwaters) 

主　　题：Na^-/K^- ratio Cynoglossus semilaevi ion-transport enzyme activity plasma osmolality growth 

摘      要：The effects of environmental Na+/K+ ratio on the gill ion-transport enzyme activity,plasma osmolality and growth of Cynoglossus semilaevis juveniles were investigated. The results showed that,plasma osmolality was similar among flounder adapted to different Na+/K+ ratios of saline groundwaters (P0.05),while the growth,gill Na+,K+-ATPase and HCO3--ATPase activities were affected by Na+/K+ ratio significantly (P0.05). The gill Na+,K+-ATPase activity reached its maximum on day 3,then decreased gradually from day 3 to day 9 and remained constant from day 9 to day 15. The peak values of gill Na+,K+-ATPase activity were detected on day 3 for all Na+/K+ ratios of saline groundwaters,then the enzyme activities descended,and on day 9 the enzyme activi-ties achieved steady state,and the gill HCO3--ATPase activity increased rapidly and achieved steady state after one day. During steady state,the gill Na+,K+-ATPase and HCO3--ATPase activity of Na+/K+ ratios 20 and 40 treatments were significantly higher than those in the control group (Na+/K+ ratio 27.5),while there were no significant differences between the Na+/K+ ratio 30 treatment and the control group; the gill Na+,K+-ATPase activity of Na+/K+ ratio 20 and 40 treatments were significantly higher than that for ratio 30 treatment,but there were no significant differences of gill HCO3--ATPase activity among these treatments. At the end of the 15-day experiment,the weight gain (%) and specific growth rate (SGR) of flounders maintained in seawater were significantly higher than those in groundwaters; significant differences also occurred among the treatments; Na+/K+ ratio 30 treatment had the highest values (33.7% and 1.94 respectively),which were significantly higher than those under Na+/K+ ratios 20 and 40 treatments. There-fore,for the saline groundwater used in this experiment,it is suggested that the Na+/K+ ratio be adjusted to approximately 30,i.e.,as close to that of natural seawater as possible in the culture of floun