Interaction of Haematopoietic Tissue Cultures of the Dublin Bay Prawn,Nephrops Norvegicus(L.),with the Causal Agent of Luminous Vibriosis Vibrio Harveyi

作     者：Mulford A.L. ZHANG X.H. XU H.S. Austin B. 

作者机构：Department of Biological SciencesHeriot-Watt UniversityRiccartonEdinburghEH144ASScotlandUK Department of Marine BiologyOcean University of QingdaoQingdao 266003P.R.China 

出 版 物：《Journal of Ocean University of Qingdao》 (青岛海洋大学学报(英文版))

年 卷 期：2002年第1卷第1期

页      面：66-70页

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：The support of European Union grant(FAIR-CI97-3413)is gratefully acknowledged 

主　　题：Vibrio haryeyi Dublin Bay prawn haematopoietic tissue interaction 

摘      要：Vibrio harveyi cells(dose103 cells mL-1)and extracellular products(ECP;25μg mL-1 of total protein concentration)destroyed haematopoietic cultures of Nephrops norvegicus within 24 h of *** effects(CPE)started after 4 h of exposure to the bacterial cells,with some granularity in the cytoplasm,mostly in cells in the outer periphe-ry of the explant *** the end of the infection,a considerable number of nuclei remained attached to the substrate,apparently *** exposure to ECP,initial deterioration was observed at 2 h with the presence of granularity in the cytoplasm of20%cells,and few cells displayed small vacuoles around the *** results were obtained using whole animal experiments,with *** cells being lethal to nephrops within 24 h.