Effects of Cytokine IL-18 Gene on Antibody Production Induced by Ag85A DNA Vaccine
细胞因子IL-18基因对Ag85A DNA疫苗诱导产生抗体水平的影响(英文)作者机构：DepartmentofUrologytheSecondHospitalofXi＇anJiaotongUniversityXi＇an710004 DepartmentofMicrobiologytheFourthMilitaryMedicalUniversityXi＇an71.0033P.R.China
出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)
年 卷 期：2004年第18卷第4期
页 面：215-218页
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
基 金：SupportedbytheNationalNaturalScienceFounda tionofChina( 3 0 170 85 5 )
主 题：mycobacterium tuberculosis Ag85A DNA vaccine IL-18
摘 要：Objective:To investigate the effects of plasmid containing human IL-18 gene on the humoral immune response of mice immunized by Ag85A DNA vaccines of Mycobacterium tuberculosis H 37R v ***: Human IL-18 cDNA was amplified from RNA of peripheral blood mononuclear cells(PBMCs)by RT-PCR and cloned into the pGEM-TEasy *** sequencing IL-18 gene was subcloned into the the sites of BamH Ⅰ and EcoR Ⅰ digestion of pcDNA3.1. BALB/c mice were injected intramuscularly with eukaryotic expression plasmid pcIL18,together with MTB pcAg85A DNA *** same immunization was repeated three times at intervals of two *** sera were collected at two weeks after the each *** titers of anti-Ag85A antibody were detected by ELISA. Results:IL-18 cDNA was amplified successfully from RNA of human PBMCs by RT-PCR and the result of sequencing was *** IL-18 gene was correctly inserted into the vector pcDNA3.1, which was confirmed with BamHⅠ and EcoRⅠ digestion analysis. The positive plasmid was called *** being immunized with DNA vaccines,the titers of antibody obtained from mice being immunized by pcAg85A combining with pcIL18 were superior to mice immunized by pcAg85A ***:Combination of IL-18 gene with MTB pcAg85A DNA vaccine could observably enhance the humoral immune responses to *** remains further investigated whether IL-18 gene plus MTB pcAg85A DNA vaccine could markedly induce the cellular mediated immune response to Ag85A or not.
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