Global epigenetic changes during somatic cell reprogramming to iPS cells

作     者：Anna Mattout Alva Biran Eran Meshorer 

作者机构：Department of GeneticsInstitute of Life SciencesThe Hebrew University of JerusalemJerusalem 91904Israel 

出 版 物：《分子细胞生物学报:英文版》 (Journal of Molecular Cell Biology)

年 卷 期：2011年第3卷第6期

页      面：341-350页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：supported by the Edmond J.Safra Foundation Israel Science Foundation (215/07 943/09) Israel Ministry of Health (6007) European Union (IRG-206872 238176) Israel Cancer Research Foundation (ICRF)and the Internal Applicative Medical Grants of the Hebrew University 

主　　题：chromatin pluripotency reprogramming iPSCs ESCs histone modifications epigenetics 

摘      要：Embryonic stem cells(ESCs)exhibit unique chromatin features,including a permissive transcriptional program and an open,decondensed chromatin *** pluripotent stem cells(iPSCs),which are very similar to ESCs,hold great promise for therapy and basic ***,the mechanisms by which reprogramming occurs and the chromatin organization that underlies the reprogramming process are largely *** we characterize and compare the epigenetic landscapes of partially and fully reprogrammed iPSCs to mouse embryonic fibroblasts(MEFs)and ESCs,which serves as a standard for *** immuno-fluorescence and biochemical fractionations,we analyzed the levels and distribution of a battery of histone modifications(H3ac,H4ac,H4K5ac,H3K9ac,H3K27ac,H3K4me3,H3K36me2,H3K9me3,H3K27me3,and gH2AX),as well as HP1a and lamin *** find that fully reprogrammed iPSCs are epigenetically identical to ESCs,and that partially reprogrammed iPSCs are closer to ***,combining both time-course reprogramming experiments and data from the partially reprogrammed iPSCs,we find that heterochromatin reorganization precedes Nanog expression and active histone ***,these data delineate the global epigenetic state of iPSCs in conjunction with their pluripotent state,and demonstrate that heterochromatin precedes euchromatin in reorganization during reprogramming.