Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis

作     者：Xiong Xiong Aixiang Hou Shuhan Yi Yichen Guo Ziwei Zhao Zhongkun Wu Huan Cheng Ke Li Zongjun Li Youhua Ren Yuanliang Wang 

作者机构：College of Food Science and Technology Hunan Agricultural University Hunan Province Key Laboratory of Food Science and Biotechnology Engineering Research Center of Union Hainan-Hunan Province Areca Nut Industry 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2018年第4卷第3期

页      面：294-299页

核心收录：

学科分类：0710[理学-生物学] 1003[医学-口腔医学] 0905[农学-畜牧学] 0906[农学-兽医学] 10[医学] 

基　　金：funded by the Hainan Province Science and Technology Major Project (No. ZDKJ2016003) the Hainan Province Applied Technology R&D and Demonstration Project (No. ZDXM2015013) the Hunan Province Xiangtan City Key Project (No. NY-ZD20161002) the Xiangtan Science and Technology Commissioner Project (No. CXY-TPZJ20171020) 

主　　题：Areca nuts Microbial diversity Oral microorganism PCR-DGGE Saliva 

摘      要：To analyze oral microbial diversity in the saliva of 8 healthy individuals before and after chewing areca nuts. Saliva samples were collected before chewing areca nuts, after chewing areca nuts for 5 min and after chewing areca nuts for 30 min. DNA was extracted, and microbial diversity was examined using PCR-denaturing gradient gel electrophoresis(PCR-DGGE). When examining DGGE profiles collectively,the bands associated with Streptococcus and Veillonella were the most intense, making them the most prevalent bacteria. Furthermore, the band intensities did not decrease after chewing areca nuts for 5 or30 min; thus, these bacteria were unaffected. However, when examining some individuals, the band intensities for Streptococcus and Veillonella became more intense after 5 min of chewing and then returned to the pre-chewing level. This difference may be attributed to the mechanical movements of the oral cavity or individual differences. Other bacteria, such as Neisseria, Actinomycetes, and Rothia dentocariosa, were also found to have an increased or decreased prevalence following areca *** the predominant species that are present following areca nut-chewing include Streptococcus and Veillonella, it would seem likely that these bacteria play an important role in the periodontal diseases associated with areca chewing.