Cytotoxicity assessment of a gold nanoparticle-chitosan nanocomposite as an efficient support for cell immobilization:comparison with chitosan hydrogel and chitosan-gelatin

作     者：Mohammad Reza RAMEZANI Hossein NADERI-MANESH Hossain-Ali RAFIEEPOUR 

作者机构：Department of BiophysicsFaculty of Biological SciencesTarbiat Modares UniversityTehranIran Department of BiotechnologyFaculty of ChemistryUniversity of KashanKashanIran 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2014年第38卷第1期

页      面：11-16页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

主　　题：gold nanoparticle-chitosan nanocomposite HEK293 cells cytotoxic effects scanning electron microscopy 

摘      要：Cell-based biosensors have become a research hotspot in the biosensors and bioelectronics fields. The main feature of cell-based biosensors is immobilization of living cells on the surface of transducers. Different types of polymers which are used as scaffolds for cell growth should be biocompatible and should have reac- tive functional groups for further attachment of biomolecules. In this work, cell attachment and proliferation on chitosan hydrogel, chitosan-gelatin and gold nanoparticle-chitosan nanocomposite membranes was stud- ied. Characterization of the membranes was performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Cytotoxicity assessment on HEK293 cells was carried out for all mem- branes using the MTT assay. Cell morphology and viability were assessed to evaluate cell attachment and pro- liferation. Regarding cell studies, the findings revealed that none of the membranes induced cytotoxic effects. However, the data showed that gold nanoparticle nanocomposite membranes improved HEK293 attachment and adhesion more than other membranes, indicating that it provides an effective surface for immobilizing cells for sensing applications.