Sequence Polymorphisms and Phylogenetic Relationships of hina Gene in Wild Barley from Tibet, China

作     者：YANG Shi-dong WEI Yu-ming QI Peng-fei ZHENG You-liang 

作者机构：Triticeae Research Institute Sichuan Agricultural University Ya＇an 625014 P..R.China Key Laboratory of Crop Genetic Resources and Improvement in Southwest China Ministry of Education/Sichuan Agricultural University Ya＇an 625014 P.R. China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2008年第7卷第7期

页      面：796-803页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Ministry of Education of the People's Republic of China, MOE Program for New Century Excellent Talents in University, NCET, (NCET-05-0814) National High-tech Research and Development Program, (200357, 2006AA10Z179, 2006AA10Z1F8) National Key Research and Development Program of China, NKRDPC, (2006 BAD13B02-06, 2006BAD01A02-23) 

主　　题：Tibetan wild barley hina SNP haplotype median-joining network 

摘      要：The hina gene encodes a HINA protein in seeds of barley （Hordeum vulgare）, which was known to affect the grain hardness. 171 hina gene sequences from Tibetan wild barley accessions and worldwide were characterized. Across 1 452 nucleotides of 171 hina genes, 152 SNPs were detected, giving an average frequency of one SNP per 9.5 bases. There were 93 singleton variable sites （the nucleotide polymorphism only observed in a single accession）, 59 polymorphic sites （the polymorphisms found in two or more accessions） and 8 indels. A total of 18 haplotypes were defined, and most of the barley accessions shared one gene haplotype. H. spontaneum had a wider haplotype distribution. Through the analysis of median-joining network of the 18 haplotypes, 4 haplotype groups were found, which were testified by neighbor-joining tree based on the complete sequence alignment. Extremely low level of hina gene diversity was observed in Tibetan wild barley accessions, indicating that Tibet is unlikely a center of origin for cultivated barley.