Correlative microscopy of Purkinje cells
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2012年第36卷第1期
页 面：1-29页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：cerebellar cells synaptic contacts electron microscopy immunohistochemistry
摘 要：The Purkinje cell and their synaptic contacts have been described using(1)light microsocopy,(2)transmission and scanning electron microscopy,and freeze etching technique,(3)conventional and field emission scanning electron microscopy and cryofracture methods,(4)confocal laser scanning microscopy using intravital stain FM64,and(5)immunocytochemical techniques for Synapsin-I,PSD9-5,GluR1 subunit of AMPA receptors,N-cadherin,and CamKII *** outer surface and inner content of plasma membrane,cell organelles,cytoskeleton,nucleus,dendritic and axonal processes have been exposed and analyzed in a three-dimensional *** intramembrane morphology,in bi-and three-dimensional views,and immunocytochemical labeling of synaptic contacts with parallel and climbing fibers,basket and stellate cell axons have been *** etching technique,field emission scanning microscopy and cryofracture methods,and GluR1 immunohistochemistry showed the morphology and localization of postsynaptic *** cell shows N-cadherin and CamKII alpha *** correlative microscopy approach provides a deeper understanding of structure and function of the Purkinje cell,a new three-dimensional outer and inner vision,a more detailed study of afferent and intrinsic synaptic junctions,and of intracortical circuits.
维普期刊数据库