Electroacupuncture exerts neuroprotective effects on ischemia/reperfusion injury in JNK knockout mice：the underlying mechanism

作     者：Chun-Xiao Wu Yi-Hui Feng Lu Yang Zhu-Lian Zhan Xiu-Hong Xu Xiao-Ying Hu Zhi-Hua Zhu Guo-Ping Zhou 

作者机构：School of Traditional Chinese Medicine Southern Medical University Guangzhou Guangdong Province China Medical College of Acu-Moxi and Rehabilitation Guangzhou University of Chinese Medicine Guangzhou Guangdong Province China Central Hospital of Qingdao Qingdao Shandong Province China Traditional Chinese Medicine-Integrated Hospital the Affiliated Hospital of Southern Medical University Guangzhou Guangdong ProvinceChina Ruikang Hospital Affiliated to Guangxi College of Traditional Chinese Medicine Nanning Guangxi Zhuang Autonomous Region China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2018年第13卷第9期

页      面：1594-1601页

核心收录：

学科分类：0710[理学-生物学] 1005[医学-中医学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100512[医学-针灸推拿学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China No.81173355 

主　　题：nerve regeneration MAPKs signaling pathway cerebral ischemia/reperfusion stress-activated pathway growth factor-activatedpathway electroacupuncture apoptosis 

摘      要：Simple regulation of c-Jun N-terminal kinase（JNK） or p38 mitogen-activated protein kinase（MAPK） pathways is not enough to trigger cell ***,activation of the stress activated pathway（JNK/p38 MAPK） together with inhibition of the growth factor activated extracellular signal-regulated kinase（ERK） pathway can promote cell *** hypothesized that inhibition of the JNK or p38 pro-apoptotic pathway and activating the ERK pathway could be the mechanism of anti-apoptosis following cerebral ischemia/reperfusion *** investigate the mechanism of the protective effect of electroacupuncture on cerebral ischemia/reperfusion injury in JNK knockout mice,mouse models of cerebral ischemia/reperfusion injury were established by Longa’s *** was conducted at acupoints Chize（LU5）,Hegu（LI4）,Sanyinjiao（SP6） and Zusanli（ST36） 1.5 hours after ischemia/reperfusion injury for 20 minutes,once a *** neurological function was evaluated using neurological deficit *** expression of phospho-extracellular signal-regulated kinase（p-ERK） and phospho-p38（p-p38） in JNK knockout mice was detected using double-labeling immunofluorescence and western blot *** m RNA expression of ERK and p38 was measured by quantitative real-time polymerase chain *** improved neurological function,increased the immunoreactivity and relative expression of p-ERK and reduced that of p-p38 in the cerebral cortex and hippocampus on the injured *** increased m RNA expression of ERK,but decreased that of p38 in the cerebral cortex and hippocampus on the injured *** conclusion,electroacupuncture upregulated the protective ERK pathway and inhibited the pro-apoptotic p38 pathway,thereby exerting a neuroprotective effect and improving the neurological function in JNK knockout mice.