Cloning and Sequence Analysis of Polygalacturonase(PG) Promoter in Tomato

作     者：Siming HOU Liying ZHOU Lulu BU Chunlei YANG Ting GAO Tian TIAN Zheng'an YANG 

作者机构：Department of Life Science and TechnologyKunming University College of Horticulture and LandscapeYunnan Agricultural University Agrotechnical Center StationAgricultural Bureau of Qiaojia County 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2016年第5卷第2期

页      面：48-50,52页

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by Natural Science Foundation of Yunnan Province(2011FB049) National Natural Science Foundation of China(31260481,31460516) Fund of Yunnan Education Department(2013Y251) Development Fund of the Department of Life Science and Technology,Kunming University(GXKM201505) 

主　　题：Tomato Polygalacturonase gene Promoter 

摘      要：In this study, 1 500 bp PG gene promoter was amplified from leaves of tomato cultivar ＇ Zhongshu No. 4＇. The bioinformatic analysis of PG promoter sequences was conducted. PG promoter elements were predicted and analyzed by PLANTCARE and PLACE. The results showed that tomato PG promoter contained multiple c/s-acting regulatory elements such as typical basic elements TATA-Box and CAAT-Box, light responsive elements 3-AF1 binding site, ATl-motif, ATCT- motif, Box 4, Box I, GA-motif, GTl-motif, Spl and MRE, heat stress-responsive element HSE, ethylene-responsive element ERE, meristem-specifie regulatory element CCGTCC-box, endosperm expression-related regulatory elements GCN4_motif and Skn-l_motif, defense and stress responsiveness element TC-rich repeats, and circadian control-related element circadian, indicating that the expression of tomato PG gene is related to light, temperature, hormone, stress and other factors. This study laid the foundation for subsequent research about regulation of PG gene expression in plants.