Effects of wrist-ankle acupuncture on associated factors in uterus tissue and serum in rats with primary dysmenorrhea

作     者：刘卫星 赵岩 虞跃跃 Liu Wei-xing;Zhao Yan;Yu Yue-yue

作者机构：Acupuncture and Tuina Department Tangshan Hospital of Traditional Chinese Medicine College of Traditional Chinese Medicine Hebei United University 

出 版 物：《Journal of Acupuncture and Tuina Science》 (针灸推拿医学（英文版）)

年 卷 期：2015年第13卷第3期

页      面：146-149页

核心收录：

学科分类：1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Hebei Tangshan Science and Technology Project No.121302118b~~ 

主　　题：Wrist-ankle Acupuncture Dysmenorrhea Acupuncture Analgesia Endorphins Nitric Oxide Prostaglandins F Substance P Rats 

摘      要：Objective：To observe effects of wrist-ankle acupuncture （WAA） onβ-endorphin （EP）, nitric oxide （NO） in uterus tissue and prostaglandin F2α （PGF2α）, substance P （SP） in serum of rats with primary dysmenorrhea. Methods：A total of 45 non-pregnant Wistar rats were randomly divided into a control group, a model group and a WAA group, 15 rats in each group. Rats in the model group and the WAA group received continuous abdominal subcutaneous injection of Diethylstilbestrol to establish dysmenorrhea rat models. On the first day after modeling, rats in the WAA group began to receive acupuncture on Point Lower 1 and Point Lower 2, once a day for 10 d. The control group and the model group didn’t receive any treatment. Writhing latencies and frequencies were recorded.β-EP and NO in uterus tissue homogenates and PGF2α, SP in serum were detected. Results：In the model group,β-EP and NO levels were the lowest among the groups, the serum PGF2α level was the highest, and serum SP level was the lowest. These measurements showed significantly difference between the model group and the control group （P〈0.05）. PGF2α in the WAA group was lower than that in the model group;β-EP, NO and SP levels were higher than those in the model group, with inter-group statistically significant differences （P〈0.05）. Conclusion： WAA may achieve analgesic effect through decreasing PGF2α, increasingβ-EP, NO and SP to relieve uterine cramps, increase blood flow and promote functional improvement.