Antioxidant and antimicrobial activities of Shorea kunstleri

作     者：Siti Suria Daud Muhammad Taher Deny Susanti Mohamed Zaffar Ali Mohamed Amiroudine Tengku Muhamad Faris Syafiq Tengku Zakaria 

作者机构：Department of Biomedical ScienceFaculty of ScienceInternational Islamic University Malaysia25200 KuantanPahangMalaysia Department of Pharmaceutical TechnologyFaculty of PharmacyInternational Islamic University Malaysia25200 KuantanPahangMalaysia Department of ChemistryFaculty of ScienceInternational Islamic University Malaysia25200 KuantanPahangMalaysia 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2014年第8期

页      面：638-644页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by International Islamic University Malaysia(Grant No.EDWB11-018-04960) 

主　　题：Shorea kunstleri Phytochemicals Antioxidant Antimicrobial 

摘      要：Objective:To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri(***)together with analysis of phytochemical and total phenolic ***:Extraction was conducted with different solvent polarity of n-hexane,dichloromethane(DCM)and methanol by using Soxhlet *** phenolic content was determined using Folin-Ciocalteu *** radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate assays,*** activities were performed using disc diffusion method,minimum inhibition concentration(MIC),minimum bactericidal concentration(MBC),and minimum fungicidal ***:*** stembark extracts revealed presence of steroids,terpenoids,saponins,flavonoids,and phenolic *** extract exhibited the highest total phenolic content and free radical scavenging activity resulting in phenolic content of(8.340±0.003)g GAE/100 g of extract and(95.90±1.07)%DPPH inhibition(IC_(50)value of 18.6μg/mL),*** thiocyanate assay of n-hexane,DCM,and methanol extracts indicated lipid peroxidation inhibitory activity of(74.20±0.35)%,(74.00±0.10)%,and(72.80±0.27)%,*** antimicrobial and antifungal tests,methanol extract showed inhibition against Staphylococcus aureus(***),Candida albicans,and Candida tropicalis with inhibition zones of 10-12,18-22,and 18-19 mm,*** MIC test of methanol extract showed highest inhibition against Candida albicans and ***(0.04 and 0.08 mg/mL,respectively)while DCM extract exhibited the highest activity towards Candida tropicalis(MIC value of 0.63 mg/mL).Taken together,MBC test of methanol extract strongly demonstrated bactericidal effect against *** with MBC value of 0.08 mg/***:The study demonstrated that stembark extracts of *** possessed antioxidant and antimicrobial properties.