Correlation of OPG/RANKL expression in gingival crevicular fluid with inflammatory factors, free radical generation and bone metabolism in patients with diabetes mellitus complicated by periodontitis

作     者：Rui Hao Fei-Fei He Yan Liang Yuan-Yuan Wei Ting-Ting Gao 

作者机构：Stomatology DepartmentXi'an No.4Hospital in Shaanxi ProvinceXi'anShaanxi Province710004 Endocrinology DepartmentXi'an No.4Hospital in Shaanxi ProvinceXi'anShaanxi Province710004 Department of Oral MedicineCangzhou Dental Hospital in Hebei ProvinceCangzhouHebei Province061000 Department of Internal MedicineNo.521Hospital of Xi'an Weapon IndustryXi'anShaanxi Province710065 Stomatology DepartmentIsmile DentalXi'anShaanxi Province710065 

出 版 物：《Journal of Hainan Medical University》 (海南医学院学报（英文版）)

年 卷 期：2018年第24卷第5期

页      面：10-13页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Scientific Research Project of Xi'an Health Bureau No: J201602037 

主　　题：Periodontitis Osteoprotegerin Receptor activator of NF-κB ligand Inflammation Free radical Bone metabolism 

摘      要：Objective:To study the correlation of OPG/RANKL expression in gingival crevicular fluid with inflammatory factors, free radical generation and bone metabolism in patients with diabetes mellitus complicated by ***:Patients with diabetes mellitus complicated by periodontitis who were treated in Xi an No. 4 Hospital between March 2015 and May 2017 were selected as the periodontitis group for the research, and healthy volunteers who underwent periodontal examination during the same period were selected as the control group. The gingival crevicular fluid was collected to determine the expression of OPG/RANKL, the contents of inflammatory factors and bone metabolism molecules as well as the generation of free ***: OPG protein expression, OPG/RANKL expression ratio as well as ICTP and Runx2 contents in gingival crevicular fluid of periodontitis group were significantly lower than those of control group whereas RANKL protein expression, TNF-α, IL-1β, IL-17, HMGB1, DKK1 and MMP1 contents as well as ROS, MDA and 8-OHdG generation were significantly higher than those of control group;OPG/RANKL expression ratio in gingival crevicular fluid of periodontitis group was negatively correlated with TNF- , IL-1β, IL-17, HMGB1, DKK1 and MMP1 contents as well as ROS, MDA and 8-OHdG generation, and positively correlated with ICTP and Runx2 ***: The excessive activation of inflammation and oxidative stress in periodontal tissue of patients with diabetes mellitus complicated by periodontitis can cause OPG/RANKL expression disorder and then lead to bone metabolism disorder.