Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE

作     者：Juan FU Yiguo JIANG Xuemin CHEN 

作者机构：Department of Occupational and Environmental Healththe Ministry of Education Key Laboratory of Environment and HealthSchool of Public HealthTongji Medical College of Huazhong University of Science and TechnologyWuhan 430030China Institute for Chemical CarcinogenesisGuangzhou Medical CollegeGuangzhou 510182China 

出 版 物：《Frontiers of Medicine》 (医学前沿（英文版）)

年 卷 期：2008年第2卷第4期

页      面：380-385页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.30571546,30771780) the Scientific Research Foundation for the Returned Overseas Chinese Scholars,The Ministry of Education of China(No.2007-24) the Natural Science Foundation of Guangdong Province(No.07117550) the Natural Science Key Program of Higher Education Institutions of Guangdong Province,China(No.06Z021) 

主　　题：anti-benzo(a)pyrene diol epoxide-deoxygua-nosine transformed cell c-Myc proteins carcinogenesis 

摘      要：Anti-benzo[a]pyrene-7,8-diol-9,10-epoxide(anti-BPDE)is a metabolite of benzo[a]pyrene(B[a]P)and acts as a potent mutagen in mammalian ***,molecular mechanisms related to anti-BPDE-induced carcinogenesis are poorly ***,we investigated the expression of proto-oncogene c-myc in human bronchial epithelial cells(16HBE-T)transformed by exposure to *** levels of mRNA and pro-tein of c-Myc were examined in the 16HBE-T and vehicle-treated control cells(16HBE-N)by using different meth-ods respectively,including reverse transcriptase-polymer-ase chain reaction(RT-PCR),quantitative real-time PCR(Q-PCR),western blot and immunocytochemical *** level of c-myc mRNA appeared to be signifi-cantly increased in 16HBE-T,as compared with those of the ***,the expression of c-Myc protein was significantly enhanced as compared with those of the control ***,the localization of c-Myc protein shows mainly nuclear staining in *** conclu-sion,the abnormal expression of c-Myc was present in anti-BPDE malignantly transformed 16HBE cells,which may be involved in the carcinogenesis molecular mech-anism of anti-BPDE.