A SPIN LABELING STUDY OF PIG CARDIAC TROPOMYOSIN
A SPIN LABELING STUDY OF PIG CARDIAC TROPOMYOSIN作者机构：Shanghai Institute of Biochemistry Academia Sinica
出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)
年 卷 期：1989年第32卷第3期
页 面：324-334页
核心收录：
基 金：Project supported by the National Natural Science Foundation of China
主 题：cardiac tropomyosin ESR 2 2 6 6-tetramethyl-4-(dichlorotriazin)-aminopiper idide-l-oxyl.
摘 要：Tropomyosin (TM) extracted from pig cardiac muscle was spin-labeled with 2,2,6,6-tetramethyl-4-(dichlorotriazin)-aminopiperidine-1-oxyl. The ESR spectra of the product (SL-TM) were of a type of weak immobilization. Effects of three means for the denaturation were observed on the above spectra. The ESR spectrum obtained for SL-TMafter enzymatic degradation was found to be analogous to that for the label itself in a dilute solution and thereby the quantity of labels bound in SL-TM estimated. The Arrhenius plots attained through variable temperature measurement for SL-TM’s exhibited two inflexion points (the conformational transition temperatures for TM) around 45℃ and 74-75℃, the latter temperature having not been reported in literature so far. However, the enzymatic degradation product from SL-TM behaved quite differently from it in the response to microwave power saturation and temperature variation.
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