Immunotoxicity Effect of Benzo[α]Pyrene on Scallop Chlamys farreri

作     者：ZHANG Lin PAN Luqing LIU Jing 

作者机构：The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2009年第8卷第1期

页      面：89-94页

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：supported by Science and Technology Development Project of Qingdao China (06-2-2-21-jch) 

主　　题：benzo[α]pyrene (B[α]P) Chlamysfarreri haemocyte counts neutral red uptake phagocytosis bacteriolytic activity antibacterial activity 

摘      要：The toxic effects of benzo[α]pyrene （B[α]P） at different concentrations （0.1, 0.5, 1, 2.5 and 7.5 μgL^-1） on scallop （Chlamys farreri） immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity （P〈0.05）, while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL^-1·B[α]P decreased significantly （P〈0.05）. Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]E Bactenolytic activity in 0.1 μgL^-1 B[α]P treatment group and antibacterial activity in 0.1 ggLl and 0.5 μgL^-1 B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group （P〈0.05）, except for antibacterial activity in 0.1 μgLl B[α]P treatment group （P〉0.05）. Thus, B[α]P has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and lmmunomodulation in aquatic organisms.