First molluscan antimicrobial peptide hydramacin in Manila clam:molecular characterization and expression analysis

作     者：Youngdeuk Lee Saranya Revathy K Sukkyoung Lee Ilson Whang Chulhong Oh Do-Hyung Kang Gee-Wook Shin Jehee Lee Mahanama De Zoysa 

作者机构：Korea Institute of Ocean Science&TechnologyAnsan426-744Republic of Korea Department of Marine Life SciencesSchool of Marine Biomedical SciencesJeju National UniversityJeju Special Self-Governing Province690-756Republic of Korea College of Veterinary MedicineChonbuk National UniversityJeonju561-756Republic of Korea Marine and Environmental InstituteJeju National UniversityJeju Special Self-Governing Province 690-814Republic of Korea College of Veterinary Medicine and Research Institute of Veterinary MedicineChungnam National UniversityYuseong-guDaejeon305-764Republic of Korea 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2014年第6期

页      面：447-452页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the National Research Foundation(NRF)program of Ministry of Education Science and Technology Republic of Korea under research grant title of the Studies on the Manila clam(Ruditapes philippinarum)internal defense system(NRF-2011-0022671) 

主　　题：Manila clam Ruditapes philippinarum Hydramacin Antimicrobial peptide 

摘      要：Objective:To investigate molecular characterization and the immune responses of Manila clam hydramacin(Rp-hdmc).Methods:cDNA sequence of hydramacin was isolated from Manila clam transcriptome *** characterization of hydramacin cDNA was performed by BLAST and SWISS-MODEL bioinformatics ***-specific expression and transcriptional regulation after Vibrio tapetis challenge was done by quantitative real time ***:Rp-hdmc has 291 bp open reading frame(ORF),encoding 97 amino acids with a mature hydramacin consisting of 77 amino acid *** un-challenged clam,Rp-hdmc was constitutively expressed in all tested tissues and the highest expression level was detected in *** pathogenic bacteria Vibrio tapetis challenge,Rp-hdmc mRNA was up-regulated in gill and ***:We identified hydramacin cDNA(Rp-hdmc)from mollusk Manila clam that shows the characteristic features of hydramacin *** has eight cysteine residues with four disulfide linkages,three helices and two β-strands in secondary *** results after *** challenges suggest that Rp-hdmc is involved in immune response against pathogenic bacteria.