Capillary method for measuring near-infrared spectra of microlitre volume liquids

作     者：YUAN Bo MURAYAMA Koichi 

作者机构：Department of Information Science and Optoelectronic Engineering State Key Laboratory of Modern Optical Instrumentation CNERC for Optical Instrument Zhejiang University Hangzhou 310027 China Department of Biochemistry and Biophysics Gifu University School of Medicine Gifu 500-8705 Japan 

出 版 物：《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 (浙江大学学报（英文版）A辑（应用物理与工程）)

年 卷 期：2007年第8卷第2期

页      面：171-175页

核心收录：

学科分类：07[理学] 070302[理学-分析化学] 0703[理学-化学] 

主　　题：Near-infrared (NIR) spectroscopy Capillary Cuvette cell Numerical analysis 

摘      要：The present study theoretically explored the feasibility of the capillary method for measuring near-infrared (NIR) spectra of liquid or solution samples with microlitre volume, which was proposed in our previous studies. Lambert-Beer absorb- ance rule was applied to establish a model for the integral absorbance of capillary, which was then implemented in numerical analyses of the effects of capillary on various spectral features and dynamic range of absorption measurement. The theoretical speculations indicated that the capillary method might be used in NIR spectroscopy, which was further supported by the empirical data collected from our experiments by comparison between capillary NIR spectra of several organic solvents and cuvette cell NIR spectra.