Microheterogeneity of acute phase proteins in patients with uIterative colitis

作     者：Marian Grzymis■awski Katarzyna Derc Magdalena Sobieska Krzysztof Wiktorowicz 

作者机构：Department of Gastroenterology and Human Nutrition University of Medical SciencesPozna■Poland Biology and Environmental Studies University of Medical SciencesPozna■Poland 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第32期

页      面：5191-5195页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Glycoproteins Acute phase response Ulcerative colitis 

摘      要：AIM： TO estimate the serum α1-antichymotrypsin （ACT）, α1-acid glycoprotein （AGP） and transferrin （Tf） concentrations and to evaluate the microheterogeneity of these acute phase proteins in patients with ulcerative colitis. METHODS： Twenty-seven patients with ulcerative colitis （UC） and 17 healthy control subjects were studied. The patients were categorised as severe （n = 9）, moderate （n = 10） and mild groups （n = 8） using Truelove and Witts＇ classification of ulcerative colitis. Microheterogeneity of ACT, AGP and Tf was analysed by crossed immunoaffinity electrophoresis （CIAE） with concanavalin A. In all serum samples standard electrophoresis of serum proteins was performed, iron （Fe） concentration, total iron binding capacity （TIBC） and C-reactive protein （CRP） were also measured. RESULTS： Our patients suffering from ulcerative colitis had significantly higher serum ACT and AGP concentrations and lower serum transferrin concentration in comparison to healthy subjects. Changes in concentrations of acute phase proteins were dependent on the activity of the inflammatory process. The glycosylation patterns of transferrin were related to the inflammation status. We also observed the correlation between ACT and AGP concentrations, patterns of transferrin glycosylation and changes in standard protein electrophoresis or blood cell count. CONCLUSION： The glycosylation patterns of transferrin obtained from patients suffering from ulcerative colitis are highly branched and sialylated compared with those obtained from healthy subjects. In contrast, the glycosylation patterns of transferrin do not differ according to the activity index of ulcerative colitis. The microheterogeneity patterns of AGP and ACT are similar in ulcerative colitis patients and healthy subjects.