Sapium ellipticum (Hochst) Pax ethanol leaf extract modulates glucokinase and glucose-6–phosphatase activities in streptozotocin induced diabetic rats

作     者：Osasenaga Macdonald Ighodaro Oluseyi Adeboye Akinloye Regina Ngozi Ugbaja Samuel Olatunbosun Omotainse 

作者机构：Department of BiochemistryCollege of BiosciencesFederal University of AgricultureAbeokuta(FUNAAB)AbeokutaNigeria Department of BiochemistryFaculty of SciencesLead City UniversityIbadanNigeria College of Vertinary MedicineFederal University of AgricultureAbeokuta(FUNAAB)AbeokutaNigeria. 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2017年第5卷第4期

页      面：162-166页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Sapium ellipticum Glucose homeostasis Diabetes Glucokinse Glucose-6-phosphatase 

摘      要：Objective: To examine the effects of Sapium ellipticum (S. ellipticum) extract on hepatic activities of glucokinase and glucose-6-phosphatase in streptozotocin (STZ)-induced diabetic Wistar ***: STZ-induced diabetic Wistar rats (four groups, n = 8) were used in this study. *** was assessed at two different doses, 400 and 800 mg/kg BW, in comparison with metformin (12 mg/kg BW) as a standard antidiabetic drug. All treatments were done orally (p.o),twice daily at 8 h interval for a period of 21 days. Glucose-6-phosphatase and glucokinase activities were respectively determined. Hepatic and muscle glycogen contents were estimated as ***: STZ caused significant decrease in glucose-6-phosphatase activity and concomitant increase in glucokinase activity. S. ellipticum especially at 400 mg/kg significantly restored glucokinase activity by 40.31% and glucose-6-phosphatase activity by 37.29%. These effects were though significantly lower than that of metformin which enhanced glucokinase activity by 94.76% and simultaneously inhibited glucose-6-phosphatase activity by 49.15%. *** also improved hepatic glycogen level by 32.37% and 27.06% at 400 and 800 mg/kg,respectively. HPLC-MS analysis of active S. ellipticum fractions in dynamic MRM mode (using the optimized compound-specific parameters) revealed among other active compounds, the presence of amentoflavone, which has been associated with anti-diabetic ***: The ability of S. ellipticum extract to concurrently inhibit glucose-6-phosphatase and activate glucokinase in this study suggests that it may be a treatment option for type 2 diabetes patients and the presence of amentoflavone in the plant extract may account for its anti-diabetic potential.