Effect of DNA damage caused by cryopreservation of spermatozoa using a modified Single cell gell electrophoresis in the freshwater catfish Pangasianodon hypophthalmus(Fowler,1936)
Effect of DNA damage caused by cryopreservation of spermatozoa using a modified Single cell gell electrophoresis in the freshwater catfish Pangasianodon hypophthalmus (Fowler, 1936)作者机构:Department of ZoologyUnit of Aquaculture and CryobiologyUniversity of Madras Guindy CampusChennai-600025TamilnaduIndia
出 版 物:《Journal of Coastal Life Medicine》 (海岸生命医学杂志(英文版))
年 卷 期:2014年第7期
页 面:515-519页
学科分类:07[理学] 070401[理学-天体物理] 0704[理学-天文学]
基 金:Supported by UGC-SAP-DSA(Grant No.SR/SO/As-03/2003)
主 题:Pangasianodon hypophthalmus Sperm Cryopreservation Genetic damage Single-cell gel electrophoresis
摘 要:Objective:To detect the integrity of sperm DNA in the catfish Pangasianodon hypophthalmus cryopreserved with Hanks balanced salt solution,5%-30%dimethyl acetamide(DMA)using the single-cell gel electrophoresis in order to test how sperm cryopreservation affected nuclear DNA ***:Electrophoresis was conducted for 60 min at 130 mA and 15 *** comet images were analyzed using software CometScore 1.5,and parameters such as comet length,tail length and percentage DNA in the tail were *** the comet rate and damage coefficient were ***:There were no significant differences in motility,comet rate and damage coefficient between fresh sperm and cryopreserved sperm stored in 5%,10%,15%and 20%DMA,while the sperm cryopreserved with 25%and 30%DMA had lower motility,higher comet length and damage coefficients than those of fresh ***:There was positive correlation between comet rate of cryopreserved sperm and the concentration of *** toxicity of cryoprotectant is the main factor for DNA damage in cryopreserved sperm.