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Effect of DNA damage caused by cryopreservation of spermatozoa using a modified Single cell gell electrophoresis in the freshwater catfish Pangasianodon hypophthalmus(Fowler,1936)

Effect of DNA damage caused by cryopreservation of spermatozoa using a modified Single cell gell electrophoresis in the freshwater catfish Pangasianodon hypophthalmus (Fowler, 1936)

作     者:Kuppusamy Umaa Rani Natesan Munuswamy 

作者机构:Department of ZoologyUnit of Aquaculture and CryobiologyUniversity of Madras Guindy CampusChennai-600025TamilnaduIndia 

出 版 物:《Journal of Coastal Life Medicine》 (海岸生命医学杂志(英文版))

年 卷 期:2014年第7期

页      面:515-519页

学科分类:07[理学] 070401[理学-天体物理] 0704[理学-天文学] 

基  金:Supported by UGC-SAP-DSA(Grant No.SR/SO/As-03/2003) 

主  题:Pangasianodon hypophthalmus Sperm Cryopreservation Genetic damage Single-cell gel electrophoresis 

摘      要:Objective:To detect the integrity of sperm DNA in the catfish Pangasianodon hypophthalmus cryopreserved with Hanks balanced salt solution,5%-30%dimethyl acetamide(DMA)using the single-cell gel electrophoresis in order to test how sperm cryopreservation affected nuclear DNA ***:Electrophoresis was conducted for 60 min at 130 mA and 15 *** comet images were analyzed using software CometScore 1.5,and parameters such as comet length,tail length and percentage DNA in the tail were *** the comet rate and damage coefficient were ***:There were no significant differences in motility,comet rate and damage coefficient between fresh sperm and cryopreserved sperm stored in 5%,10%,15%and 20%DMA,while the sperm cryopreserved with 25%and 30%DMA had lower motility,higher comet length and damage coefficients than those of fresh ***:There was positive correlation between comet rate of cryopreserved sperm and the concentration of *** toxicity of cryoprotectant is the main factor for DNA damage in cryopreserved sperm.

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