Reexamining intra and extracellular metabolites produced by Pseudomonas aeruginosa

作     者：Maria Shuja Nazia Jamil 

作者机构：Department of Microbiology and Molecular Genetics University of the PunjabLahore-54590Pakistan 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2016年第4卷第2期

页      面：132-139页

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：Supported by International foundation for Science IFS Sweden(Grant No.F/4739-2F)and University of the Punjab Lahore Pakistan 

主　　题：Antimicrobial activity Solubilization Zinc Oxide Zinc Phosphate Bioplastics 

摘      要：Objective:To isolate,screen and analyze bacteria from different areas of Pakistan for the production of antimicrobial compounds,zinc solubilization and bioplastic ***:Isolation and purification was proceeding with streak plate *** assay was completed with well diffusion and thin-layer *** vivo analysis of bioplastic was analyzed with Nile blue fluorescence under UV and Sudan ***:A total of 18 bacterial strains purified from soil samples while 148 strains form stock cultures were *** of 166 only 94 showed antimicrobial activity against each of Grampositive and Gram-negative;cocci and *** case of heavy metal(ZnO and Zn_(3)(PO_(4))_(2)·4H_(2)O)solubilization,54 strains solubilized ZnO and 23 strains solubilized Zn_(3)(PO_(4))_(2)·4H_(2)O,while 127 strains grown on polyhydroxyalkanoate detection meedia supplemented with Nile blue medium showed bioplastic production by producing fluorescence under UV *** bacterial strains(coded as 100,101,104 and 111)were selected for further *** time assay showed that strains 101,104,and 111 showed inhibitory activity after 4 h of incubation while strain 100 showed after 8 *** four strains were tolerable to the maximum concentration of *** products of both 16S rRNA and PhaC gene fragments of strain 111 were sequenced and submitted to GenBank as accession numbers EU781525 and ***:Bacterial strain Pseudomonas aeruginosa-111 has potential to utilize as biofertilize and bioplastic producer.