Ecological distribution of harmful epiphytic Oscillatoriales in Alexandria coast,Egypt,with special reference to DNA identification

作     者：Amany Abdel Hamid Ismael Eman Abdel Razak Mohamed Mostafa Mohamed El-Sheikh Wafaa Hassan Hegazy 

作者机构：Oceanography DepartmentFaculty of ScienceAlexandria UniversityAlexandria 21511Egypt Botany DepartmentFaculty of ScienceDamanhuor UniversityEgypt Botany DepartmentFaculty of ScienceTanta UniversityTantaEgypt 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2014年第4期

页      面：274-280页

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Supported by University of Tanta(Grant No.2009/2013) 

主　　题：Harmful algae DNA analysis epiphytes Oscillatoriales Alexandria Egypt 

摘      要：Objective:To identify the potentially harmful epiphytic Oscillatoriales species and follow up their distribution along Alexandria ***:Samples were collected bimonthly from April 2009 to February 2010 at three sites along Alexandria *** morphological and molecular analyses were used for identifying the dominant ***:Five species belonging to two families were identified;Oscillatoria acutissima,Oscillatoria nigroviridis,Oscillatoria sp.,Lyngbya majuscule and Phormidium *** cell density ranged from 10^(3)to 126×10^(3)filament g^(-1)fresh weight *** morphological study of the dominant species,Oscillatoria sp.(Oscillatoria sp.W1)showed much similarity with Planktothrix agardhii with no heterocysts and akinetes,while molecular ananlysis(16S rDNA)clustered the species in the same group with Anabaena ***:The 16S rDNA genes are not suitable for identifying Oscillatoriales during the present study and another molecular method should be used instead.